ISSN: 2582-788X (Online)
Isolation and characterization of bacteria of Amjhupi and Ishurdi soil series of Bangladesh
Bacteria are single celled, ancient life form, omnipresent and living in all conditions. Its isolation and identification from different resources is very much important for its classification and knowledge of its pathogenicity. In soils the bacteria and fungi dominated by its presences and account for almost all the biological and chemical changes in environments. In this study, bacteria from Amjhupi and Ishurdi, Bangladesh soil series were isolated and characterized. Well-isolated colonies of bacteria were evaluated in size, pigmentation, form, margin and elevation. It has been observed that Ishurdi soil was found to have more bacterial population than that of Amjhupi soil. From simple and negative staining, shape and arrangement were determined. The ratio of Gram- positive bacteria to Gram- negative bacteria was evaluated for both soils. Majority of the bacteria were spore and capsule former. The bacteria were non-acid fast and were arranged in chain form in both soils. The presence of rod shaped bacteria (bacillus) compared to round shaped (coccus) in soils is conspicuous.