Enumeration, isolation and characterization of bacteria of Gopalpur soil series of Bangladesh

For enumeration, isolation and characterization of bacteria soil sample from Gopalpur soil series was collected. The counted bacterial population was as 4.5×107 CFU/g soil. Moreover, colony characteristics as well as morphological characteristics were studied with an intensive care. Well-isolated colonies of nutrient agar plates were evaluated in size, pigmentation, form, margin and elevation. Staining characteristics were determined by Gram stain, capsule stain, spore stain and acid fast stain methods. Most of the bacteria of this soil were found as rod shaped. All types of bacteria were available as non-acid fast. Gram- positive bacteria were prominent where Gram-negative, spore forming, non-spore forming; capsulated and non- capsulated bacteria were observed.